Part:BBa_K1412829
Characterize efficiency of RBS with chemotaxis
BBa_K1412829: Plac-RBS(0.3)-CheZ-TT
This part consists of [http://en.wikipedia.org/wiki/Chemotaxis CheZ] gene which can express CheZ protein make E.coli tumble or swim straight. In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS). Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.
Usage
When we want to characterize the efficiency of RBS, we usually connect the RBS with GFP, and then measure the fluorescence intensity of GFP. In our part, you only need to connect RBS after a Plac promoter and before CheZ gene, ending with a double terminator(Plac-RBS(changeable)-CheZ-TT). Then transfer this gene circuit into E.coli CL-1 (CheZ knocked out), and coat plates, culture on semi-solid medium to measure the migration diameter of E.coli.
Relevant parts
BBa_K1412000: pLac-RBS(1.0)-CheZ-TT CheZ generator under pLac promoter
BBa_K1412005: RBS(1.0)-CheZ-TT
BBa_K1412006: RBS(0.01)-CheZ-TT
BBa_K1412007: RBS(0.3)-CheZ-TT
BBa_K1412014: pTet-RBS(1.0)-CheZ-TT Characterize the efficiency of promoters with chemotaxis
BBa_K1412614: pBAD-RBS(1.0)-CheZ-TT Characterize the efficiency of promoters with chemotaxis
BBa_K1412801: pLac-RBS(0.01)-CheZ-TT Characterize the efficiency of RBS with chemotaxis
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]
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