Translational_Unit

Part:BBa_K1497002:Design

Designed by: Sascha Hein, Sven Jager, Thomas Dohmen, Sven Rumpf   Group: iGEM14_TU_Darmstadt   (2014-10-07)
Revision as of 20:05, 17 October 2014 by SaHein (Talk | contribs) (→‎Source)

B0034-eANS (engineered anthocyanidin synthase with strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For improving the enzyme the iGEM team TU Darmstadt 2014 decided to remove the tail and to construct their pelargonidin operon with this engineered ANS (eANS). The laboratory results cover the previous modeling results. The engineered ANS exhibited better yields than the original one when used in an operon producing pelargonidin (BBa_K1497015).


Gene was E. coli optimated synthezied by Eurofins.


Source

Fragia x anassana

References