Composite

Part:BBa_K1413023:Design

Designed by: Laura MATABISHI, Nandjafot MENDY   Group: iGEM14_Evry   (2014-10-17)
Revision as of 19:32, 17 October 2014 by Registry (Talk | contribs)

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Promoter-RBS-bphr2 mutated-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352


Design Notes

Between each part, there is the scar SpeI/xbaI. Moreover, bphr2 gene has been mutated.


Source

J23114, B0034 and terminator come from the 2014 kit distribution bphr2 has been synthetized (it is the part BBa_K1413021)

References