Generator

Part:BBa_K1343016

Designed by: Karen Joseph   Group: iGEM14_Technion-Israel   (2014-10-05)
Revision as of 19:17, 17 October 2014 by Rebeccaf77 (Talk | contribs)

Pcat->RBS->TaZ->dTer

This part generates the TaZ receptor. The TaZ is under a Pcat constitutive promoter. TaZ is a fused Tar-EnvZ receptor meant for Aspartate dependent response, by activating the EnvZ/OmpR two component signalling system and thus activating the PompC promoter. We used this part for prove-of-concept for low concentration detection. By using this TaZ Generator we were able to test different concentrations of Aspartate and their effect on the PompC promoter present in another plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 167


[edit]
Categories
//classic/signalling/sender
Parameters
None