Composite
Part:BBa_K1462280
Designed by: Chuyao Fan Group: iGEM14_SCUT (2014-10-10)
Gal1+cox6+Crt+cyc1
This part is constructed as a control group to BBa_K1462130 to identify Crt has been directed to the mitochondria. The GFP is fused at the C-terminus of Crt to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |