Composite

Part:BBa_K1462280

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)
Revision as of 19:15, 17 October 2014 by Fcy (Talk | contribs)

Gal1+cox6+Crt+cyc1

This part is constructed as a control group to BBa_K1462130 to identify Crt has been directed to the mitochondria. The GFP is fused at the C-terminus of Crt to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None