Coding

Part:BBa_K1510010:Experience

Designed by: Chien-Yung Huang   Group: iGEM14_NYMU-Taipei   (2014-10-06)
Revision as of 18:37, 17 October 2014 by Tree1220 (Talk | contribs) (Applications of BBa_K1510010)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1510010

Secreting red fluorescence protein (RFP) extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.

User Reviews

UNIQb5c8088633e15422-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg UNIQb5c8088633e15422-partinfo-00000001-QINU