Coding
INT-B0034

Part:BBa_K1433021:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-16)
Revision as of 17:44, 17 October 2014 by Lilina (Talk | contribs) (→‎References)

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Promoter-B0034-gp35-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 513
    Illegal XhoI site found at 600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1152
    Illegal NgoMIV site found at 1239
    Illegal AgeI site found at 289
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1347


Design Notes

no


Source

Derive from 2014 Distribution Kit.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.