Coding
REINT-6N

Part:BBa_K1433017:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)
Revision as of 17:39, 17 October 2014 by Lilina (Talk | contribs) (→‎References)

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P-C-attL-rT-rT-attR-B-6N-gp35-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 457
    Illegal NheI site found at 654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 928
    Illegal XhoI site found at 1015
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 1567
    Illegal NgoMIV site found at 1654
    Illegal AgeI site found at 704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99
    Illegal BsaI.rc site found at 357
    Illegal BsaI.rc site found at 1762


Design Notes

no


Source

Derive from 2014 Distribution Kit and synthesis by Genewiz..

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.