Composite

Part:BBa_K1498001:Design

Designed by: Charlotte Green   Group: iGEM14_Sheffield   (2014-10-07)
Revision as of 17:36, 17 October 2014 by Charlieinabox (Talk | contribs) (→‎Design Notes)

constitutive promoter with lipase (BBa_J23110, BBa_K258006)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli.

Source

Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue, BBa_J23110 was retrieved from the distribution kit

References

Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11