Coding
REINT-B003

Part:BBa_K1433016:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)
Revision as of 17:36, 17 October 2014 by Lilina (Talk | contribs) (→‎References)

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P-C-attL-rT-rT-attR-B-B0031-gp35-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 917
    Illegal XhoI site found at 1004
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 1556
    Illegal NgoMIV site found at 1643
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99
    Illegal BsaI.rc site found at 357
    Illegal BsaI.rc site found at 1751


Design Notes

no


Source

Derive from 2014 Distribution Kit and synthesis by Genewiz.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.