Part:BBa_K1498001

constitutive promoter with lipase (BBa_J23110, BBa_K258006)

Composite comprises of the constitutive promoter (BBa_J23110) with Thermostable lipase (tliA) of Pseudomonas fluorescens SIK W1 (BBa_K258006).

Four glycine-rich repeats (GGXGXD) in the tliA C-terminus, allow ATP binding cassette (ABC) transporter secretion of the lipase (Chung et al., 2009). ABC transportation results in the movement of protein through the inner and outer membrane of gram negative bacteria.

References.

Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 692
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]