Coding
Suppor Dev

Part:BBa_K1433013:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)
Revision as of 17:28, 17 October 2014 by Lilina (Talk | contribs) (→‎References)

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rT-rRFP-rRBS-attB-P-attP-RBS-lambda red-RBS-GFP-T-P-RBS-gp47-tag-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 926
    Illegal NheI site found at 949
    Illegal NheI site found at 4991
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4930
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 873
    Illegal NgoMIV site found at 5025
    Illegal AgeI site found at 2429
    Illegal AgeI site found at 4765
    Illegal AgeI site found at 5793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 893
    Illegal BsaI.rc site found at 976
    Illegal BsaI.rc site found at 3573
    Illegal SapI site found at 4747


Design Notes

no


Source

Derive from 2014 Distribution Kit and pKD46 plasmid.

References

  1. Baba T, Ara T, Hasegawa M, et al. Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection[J]. Molecular systems biology, 2006, 2(1).
  2. Mosberg J A, Lajoie M J, Church G M. Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate[J]. Genetics, 2010, 186(3): 791-799.
  3. Datsenko K A, Wanner B L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products[J]. Proceedings of the National Academy of Sciences, 2000, 97(12): 6640-6645.
    4. li>Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  4. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.
  5. Sharan S K, Thomason L C, Kuznetsov S G, et al. Recombineering: a homologous recombination-based method of genetic engineering[J]. Nature protocols, 2009, 4(2): 206-223.