Composite

Part:BBa_K1433006:Design

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 17:11, 17 October 2014 by Lilina (Talk | contribs) (→‎References)

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B0031-gp35-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 480
    Illegal XhoI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 1206
    Illegal AgeI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1314


Design Notes

No


Source

Derive from 2014 Distribution Kit.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.