Part:BBa_K1336003:Experience

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Applications of BBa_K1336003

Results

Degradation

Azo-sensor

Biosafety

Degradation

After having confirmed that Reactive Black 5 and Acid Orange 7 are not toxic and have no effect of ''E. coli'' DH5α in a wide range of concentrations, we set out to determine how the dye-decolorizing BioBrick BsDyP BBa_K1336003 affected E. coli growth performance, both in standard LB medium and in media contaminated with RB5 and AO7 sulphonated azo-dyes.

This was carried out by measuring bacterial OD at 680nm at regular intervals of 1 hour, in the different media. The choice of wavelength aims to reduce to a minimum the interference caused by the strong absorption of the dyes, while still measuring bacterial density. Although high-concentration RB5 still shows an absorption much higher than the other samples, the growth curve is not affected and so it allows to analyse how the presence of dyes might interfere with bacterial growth. The full protocol fot this assay can be found here (insert link).



Figure 1 - BBa_K1336003 BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.


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