Translational_Unit

Part:BBa_K1497012

Designed by: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky   Group: iGEM14_TU_Darmstadt   (2014-10-07)
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B0034-F3H

This part encodes the sequence for the enzyme Flavanone-3ß-hydroxylase (F3H) EC 1.2.7.3 from Petroselinum crispum behind the strong RBS B0034. F3H catalyze naringenin, an anthocyanidin precursor, to the substance dihydroflavonol (Tanaka, 2006). The enzyme have 368 amino acids and a molecular weight of 41kDa.


Figure 1 Reaktion of the F3H.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


=References

1. Tanaka Y, Brugliera F, B PTRS, et al. (2013) Flower colour and cytochromes P450 Flower colour and cytochromes P450 †.

[edit]
Categories
//cds/biosynthesis
//cds/biosynthesis/anthocyanins
Parameters
n/aB0034-F3H