Composite

Part:BBa_K1431301:Design

Designed by: Rifei Chen, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-10)
Revision as of 13:42, 17 October 2014 by ChenRifei (Talk | contribs) (→‎Design Notes)


TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For BbsI site, we have a series same design sequence. And the reason why we designed that because we want to make coding sequence seamless gather with promoter and PolyA or any amount nucleotides. That depend on which will make the highest efficiency. Sorry for the limited time, we only test GFP seamless gather with promoter and PolyA but did not get the data which will make the highest efficiency.

Source

It is from our instructor's lab.

References