Part:BBa_K1462090:Design

Gal1+cox6+GFP+ADH1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 150
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1325
  

Design Notes

To verify the localization function of CoxVI, the C-terminus of CoxVI was fused with GFP. We adopted fluorescence test to have a visible look at where the GFP is located. The following microscope images were captured(Figure 1). We can observe that the green fluorescence concentrates in a specific area , instead of diffusing homogeneously in the cytosol.The reult proves that CoxVI can perform its function properly [[File:|200px|left|frame|Figure 1.COXVI function verification GFP fused with COXVI is concentrated in a specific area of the cytoplasm,while GFP without COXVI diffuses in the cytoplasm of the cell. ]]