Coding

Part:BBa_K1321113

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 11:52, 17 October 2014 by Xen sm (Talk | contribs)

Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter

A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG


Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 512
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 512
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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