Coding

Part:BBa_K1331001:Design

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-25)
Revision as of 11:26, 17 October 2014 by Danielle D (Talk | contribs) (→‎Design Notes)

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Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478


Design Notes

A mutation has been done to remove the PstI restriction site contained in the coding sequence without changing the amino acid sequence.

Source

Pseudomonas aeruginosa SQ6

References