Coding

Part:BBa_K1331001

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-25)
Revision as of 11:09, 17 October 2014 by Danielle D (Talk | contribs)

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit A.RhlA together with rhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. It includes the whole coding sequence and a part of downstream noncoding gene of RhlA. The coding sequence is similar withPart:BBa_K317998[1] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None