Part:BBa_K1431411:Design

target sequence for HIV

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

After we use design the gRNA of HIV-1(BBa_K1431401), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .

But there are two problem:

First,the DSB of binding sequence is hard to observe in the cell;

Second,the whole HIV genome have a potential dangers in the lab.

So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of binding sequence to the change of reporter. The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HIV genome.

Source

HIV1_REF_2010_genome_DNA