RNA

Part:BBa_K1431402

Designed by: Fan Jiang, Peng Peng   Group: iGEM14_SUSTC-Shenzhen   (2014-10-14)
Revision as of 09:11, 17 October 2014 by Brando (Talk | contribs)

gRNA1 for HBV

This is a gRNA1 for the HBV Virus, designed by the 2014 iGEM Team SUSTC-Shenzhen.

Here, we engineer the type II CRISPR system to come true double strands break with gRNA in hela cells.We show that this process relies on CRISPR two specificity components:gRNA and its binding target-sequence.

In this part, we put on a high-specificity gRNA of HBV with more detail in design.

In addition ,this part use efficient BbsI restriction site for easily used.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None