Composite

Part:BBa_K1462220:Design

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)
Revision as of 09:11, 17 October 2014 by Weapon (Talk | contribs)

Gal1+Hbd+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 942


Design Notes

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.Hbd,originated from Clostridium beijerinckii, expresses 3-hydroxybutyryl-CoA dehydrogenase, which converts AcAcCoA to HbCoA, the second step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Erg10(BBa_K1462210),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph. 100px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.