Translational_Unit

Part:BBa_K1500010

Designed by: Rivka Baker Keusch   Group: iGEM14_UChicago   (2014-10-17)
Revision as of 08:24, 17 October 2014 by RBeckner (Talk | contribs)

IPTG--GFP--EmrR

Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR and mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 1.13E-08 mutations per cell per culture as compared to 2.84E-09 mutations per cell per culture for wild-type XL1Blue. This plasmid appears to incur a growth penalty in transformants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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Categories
Parameters
None