Part:BBa_J01008:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ82c236ebb380d640-partinfo-00000000-QINU UNIQ82c236ebb380d640-partinfo-00000001-QINU

••••• HZAU-China 2014

We want to know under the participation of taRNA and crRNA, whether can reduce leakage of lac promoter’s expression. So we did some experiment to text. First, built the tow devices and from top to bottom Numbers for 1 2 , and then, respectively transform the three plasmid into BL21 competent cell. Pick monoclonal colony. Add 5ml LB liquid and incubate at 37 Celsius degree for 8 hours. Secondly, add 5ul of E.coli liquid cultural medium from each tube to two new tubes named A, B respectively with 5ml M9 broth in it. Add 0.1ul IPTG to all A tubes (1A, 2A, ) while add nothing to all B tubes (1B, 2B, ). Incubating at 37 Celsius degree for 4 hours. Lastly, easuring the fluorescent degree by utilizing the enzyme-labeled instrument. Conclusion : riboregulators ensure much lower leakage of the promoters.