Plasmid

Part:BBa_K1391012

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 05:52, 17 October 2014 by Registry (Talk | contribs)

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pENTR_Cofilin (Inactivated - pseudophosphorylated)

Cofilin is an actin depolymerizing factor. It is recruited when PirB/LilrB2 is activated by beta-amyloid. [1]

The 3rd amino acid in this construct has been mutated to make it pseudophophoralated and inactive. It was ordered from Adgene.

[1] Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. <http://www.sciencemag.org/content/341/6152/1399>. 16 Oct 2014.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 268
    Illegal SapI site found at 108


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Categories
Parameters
None