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Part:BBa_K1420000

Designed by: Jessica Tarnowski   Group: iGEM14_Minnesota   (2014-10-08)
Revision as of 05:09, 17 October 2014 by Valeriubortnov (Talk | contribs) (→‎Components of the mer operon)

Mer operon, biological system found to detoxify organic and inorganic forms of mercury

converts organomercuric and cationic mercury to the less toxic volatile mercury.

Components of the mer operon

merR


merT


merP


merA

Mercury resistance gene merA encodes MerA, a mercuric ion reductase (cytosolic, flavin disulfide oxidoreductase), that is essential to bacterial mercury resistance. MerA catalyzes the reduction of mercuric ion released from protonlysis of organomercury (catalyzed by MerB) to the relative inert, volatile monoatomic mercury in a NADPH dependent reaction. (For more information on the merA gene, see part BBa_K1420001.)


merB

The merB gene is often found immediately downstream of merA, and is essential for the detoxification and bioremediation of organic toxic mercury compounds in congruence with merA. The merB protein is a lyase that catalyzes the breaking of carbon-mercury bonds through protonolysis of toxic mercury compounds, such as methylmercury. This produces the less toxic and less mobile Hg2+ which is then completely volatilized to Hg0 when acted upon the enzyme merA. (For more information on the merB gene, see part BBa_K1420002.)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 551
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2420
    Illegal NgoMIV site found at 2468
    Illegal NgoMIV site found at 2530
    Illegal NgoMIV site found at 2741
    Illegal NgoMIV site found at 3334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 536


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