Coding
BtGal1

Part:BBa_K1489001

Designed by: Iowis Zhu   Group: iGEM14_UMaryland   (2014-10-06)
Revision as of 04:48, 17 October 2014 by HHeLiBeBCNOFNe (Talk | contribs)

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Bovine Galectin-1

His-tagged soluble beta-galactoside binding protein (galectin) originally from Bos taurus. BtGal1 is believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. It is believed to be a dimer in its active form, as is true with most mammalian galectins.

UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None