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Coding

Part:BBa_K1323010

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)
Revision as of 04:37, 17 October 2014 by Rkovac31 (Talk | contribs)

YFP Coding Sequence

This construct contains the reporter protein coding sequence for the yellow fluorescent protein (YFP). It has been codon optimized for Staphylococcus aureus by Bose et al. (2013). YFP has an excitation peak at 514 nm and an emission peak at 527 nm (needs ref). We order the synthesis of this coding sequence from Biobasic. This is a reliable reporter in S.aureus (Malone et al., 2009).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.


[edit]
Categories
//cds/reporter/yfp
//function/reporter/fluorescence
Parameters
colorYellow