Regulatory

Part:BBa_K1509003:Design

Designed by: Tong Li   Group: iGEM14_NEFU_China   (2014-09-26)
Revision as of 04:28, 17 October 2014 by LiTong (Talk | contribs)

CP25 is a highly active,constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CP25 was used as the strong constitutive promoter to regulate the expression of the gene CDS7.


Source

We synthesized CP25 from a library of synthetic promoters for Lactococcus lactis.

References

1. Jensen, P.R. and K. Hammer, The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl Environ Microbiol, 1998. 64(1): p. 82-7.