Part:BBa_K1323016

Translational initiation region (RBS) for Staphylococcus aureus

The Translation Initiation Region ribosome binding site (TIR rbs) was isolated from T7 bacteriophage (Fey, 2014). It is used for optimal expression in s.epidermidis when a native RBS is too poor to achieve desired expression levels. It has a Shine-Dalgarno consensus sequence (AGGAGG). The start codon and the phage T7 enhancer were spaced to optimally minimize secondary structure formation (Cheng et al., 1992). The TIR RBS leads to greater expression levels compared to sodA RBS BBa_K1323022 even though they have the same consensus sequence (Fey, 2014).

References

Cheng, X., Patterson, T. A., (1992) Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res 20, 4591–4598.

Fey, P. D. (2014). Staphylococcus epidermidis: methods and protocols. New York: Springer Science + Business Media, LLC.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]