Coding

Part:BBa_K1323013

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)
Revision as of 04:15, 17 October 2014 by Stenhold (Talk | contribs)

aadD - Kanamycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic kanamycin. The sequence of this gene is obtained from pKAN vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The working concentration of kanamycin is 250ug/mL for aadD expressed on a plasmid and 75ug/mL for aadD on chromosome (Bose et al., 2013)


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 561
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 385


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
None