Part:BBa_K1323005:Experience

sRNAi Silencing assay

YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.

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