Part:BBa_K1323004

Hfq: Expression cassette under a xylose inducible promoter

Hfq is a protein which enhances sRNA complementation to its mRNA target - It does so by binding AU-rich and poly-A-rich sequences; Hfq-dependent sRNAs have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.

This part was DNA-Synthesized.

References

Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocol 8(9), 1694-1707

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1476
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]