Coding
Part:BBa_K1323003
Designed by: Julia Manalil Group: iGEM14_Waterloo (2014-09-04)
oriT-Nes: expression sequence for Staphylococcus conjugation
oriT-Nes is a 2.2kb region that includes an origin of transfer (oriT) and a gene that encodes the oriT-nicking protein (Nes). This sequence originates from the staphylococcal plasmid, pGO1 (Caryl & O’Neill, 2008). When a plasmid in a gram-positive organism undergoes conjugation, a multiprotein complex binds at the origin of transfer to form a relaxosome. The relaxosome is recognized by DNA relaxases that perform a cut in DNA which readies the DNA for transfer from the cell (Grohmann et al, 2003). Our team obtained the pGO1 plasmid from Dr Alex O’Neill’s lab at the University of Leeds. We amplified the oriT-Nes region out of pGO1 by PCR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |