Coding

Part:BBa_K1323003

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-09-04)
Revision as of 00:37, 17 October 2014 by Stenhold (Talk | contribs)

oriT-Nes: expression sequence for Staphylococcus conjugation

oriT-Nes is a 2.2kb region that includes an origin of transfer (oriT) and a gene that encodes the oriT-nicking protein (Nes). This sequence originates from the staphylococcal plasmid, pGO1 (Caryl & O’Neill, 2008). When a plasmid in a gram-positive organism undergoes conjugation, a multiprotein complex binds at the origin of transfer to form a relaxosome. The relaxosome is recognized by DNA relaxases that perform a cut in DNA which readies the DNA for transfer from the cell (Grohmann et al, 2003). Our team obtained the pGO1 plasmid from Dr Alex O’Neill’s lab at the University of Leeds. We amplified the oriT-Nes region out of pGO1 by PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None