Device

Part:BBa_K1420006

Designed by: Stephen C. Heinsch   Group: iGEM14_Minnesota   (2014-10-09)
Revision as of 23:48, 16 October 2014 by Valeriubortnov (Talk | contribs) (Experimental Results)

phsABC Generator

Summary

The (phsABC) gene from Salmonella enterica serovar Typhimurium LT2 encodes thiosulfate reductase, which catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate for heavy metal removal by precipitation.

Experimental Results

In order to measure the phsABC conversion rate of NaS2O3 to H2S, the gene was inserted into the pBBRBB vector with a constitutive Plac promoter and transformed into K12 strain E. coli cells. As a control, pBBRBB::GFP was tested under the same conditions. The pBBRBB:phsABC K12 and pBBRBB::GFP K12 cells were grown in a test tube containing heavy metal tryptone medium as well as 3mM NaS2O3.

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
    Illegal BglII site found at 2197
    Illegal BamHI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
    Illegal AgeI site found at 2772
    Illegal AgeI site found at 3555
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None