RNA

Part:BBa_K1323000

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-07)
Revision as of 23:43, 16 October 2014 by Stenhold (Talk | contribs)

sgRNA (1): CRISPR Silencing of YFP

Single guide RNA1 is an RNA oligo that binds to dCAS9 (BBa_K1323002) enabling the complex to bind to the coding strand of the YFP ORF (ccatctaattcaaccaaaat). sgRNA is a fusion of tracrRNA (trans-activation crRNA) and crRNA (CRISPR RNA), where the 20bp sequence complementary to the target sequence is linked to a hairpin loop that is similar to tracrRNA. When sgRNA-dCas9 bind to the target sequence, it is expected to act as a programmable repressor and silence the expression of the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None