Part:BBa_K1541017

rhlI with optimized RBS

N-butyryl-L-Homoserine lactone or short C4-HSL

This part contains the part C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR (C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.

Usage and Biology

Quorum Sensing

RhlI is the autoinducer synthetase from Pseudomonas aeruginosa, which can synthesize the molecule N-butyryl-L-HSL (C4-HSL). C4-HSL can bind to the regulatory protein RhlR (C0171). Once C4-HSL is bound to RhlR it can activate the promoters R0071 or I14017.

RBS Engineering

The ribosomal binding site (RBS) is a sequence on the mRNA crucial for the translation initiation step in prokaryotes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]