Coding

Part:BBa_K1418062:Design

Designed by: Ryan Putman   Group: iGEM14_Utah_State   (2014-10-16)
Revision as of 22:21, 16 October 2014 by Ryanputman (Talk | contribs) (→‎Design Notes)

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GFPmut


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was maintained after mutagenesis. Stop codon was removed for in frame protein fusions.

Source

GFPuv

References