Coding

Part:BBa_K1493601

Designed by: Teresa Robert Finestra   Group: iGEM14_Wageningen_UR   (2014-10-06)
Revision as of 22:08, 16 October 2014 by Trobert (Talk | contribs)

Kis Antitoxin (Kis-Kid Toxin-Antitoxin System)

Kid antitoxin.

Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. In our system an excess of Kid will result in a bactericidal effect. Additionally, the Kis-Kid interaction has proven to be a 1:2 ratio [5, 6].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None