Reporter
rev RFP

Part:BBa_K1463520

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-12)
Revision as of 22:08, 16 October 2014 by Scolloms (Talk | contribs) (→‎Usage and Biology)

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E1010 RFP in reverse

Part E1010, RFP, in reverse.

Usage and Biology

This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site BBa_K1463560 with transcription driven by the reverse promoter BBa_K1463501 in BBa_K1463000.

See BBa_K1463560 and BBa_K1463000 for more details.

Fig. 1: Red fluorescence of colonies containing BBa_K1463560 or BBa_I742130 reversed RBSs "downstream" of the reversed rfp gene BBa_K1463520 transcribed from the PhiC31 integrase switch BBa_K1463000. The image was scanned using a GE Healthcare Typhoon FLA9500 using the 532 nm laser and LPG filter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None