Regulatory

Part:BBa_K1545001

Designed by: Mike Zhu   Group: iGEM14_Duke   (2014-10-09)
Revision as of 21:34, 16 October 2014 by Mzhu22 (Talk | contribs)

6xGFP1 decoy

Array of 6 binding sites for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer.

Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below:

BiggerDecoyReflow.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None