Part:BBa_K1317001:Design
CDS for resilin-like polypeptide (RLP)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 300
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene used to assemble the biobrick has been provided by GenScript even if the assembling strategy succeeded
It allows us to have the proper sequence without mutation and with sequencing results.
The strategy of our assembly is described below anyway. The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for further fusing proteins.
Source
Genomic sequence from Drosophila melanogaster
References
Julie N. Renner et al. Characterization of Resilin-Based Materials for Tissue Engineering Applications Biomacromolecules 2012, 13, 3678−3685
Russell E. Lyons et al. Comparisons of Recombinant Resilin-like Proteins: Repetitive Domains Are Sufficient to Confer Resilin-like Properties Biomacromolecules 2009, 10, 3009–3014
M. Kim et al. High yield expression of recombinant pro-resilin: Lactose-induced fermentation in E. coli and facile purification Protein Expression and Purification 52 (2007) 230–236
Linqing Li and Kristi L. Kiick Resilin-Based Materials for Biomedical Applications ACS Macro Lett. 2013, 2, 635−640