Coding

Part:BBa_K1378003

Designed by: Meng Liuyi & Zhang Zijian   Group: iGEM14_Peking   (2014-10-03)
Revision as of 16:42, 16 October 2014 by Purord.z (Talk | contribs)

INPNC-linker2-MVN-linker1-mRFP

Introduction

This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Microcystis aeruginosa PCC7806 specifically.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1029

Usage and Biology

<figure><img src="Peking2014ZZJ_MVN.png"

/><figcaption>Figure 2. Solution structure of MVN. MVN consists of 2 domains, domain A

and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is

formed by residues 1-37 and 94-108. The mannan binding site is only found in domain A. This figure is taken from

Protein Data Bank (PDB ID: 2Y1S).</figcaption></figure>



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