Terminator

Part:BBa_B0013

Designed by: Reshma Shetty   Group: Antiquity   (2003-01-31)
Revision as of 15:45, 22 April 2006 by Randy (Talk | contribs)

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Designed by Reshma Shetty Entered: 2003-01-31

TE from coliphageT7

Bidirectional transcriptional terminator consisting of a 22bp stem-loop

Usage and Biology

Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the stem loop. It is also found between two coding regions that point towards each other.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyT7TE
directionForward
forward_efficiency0.309[CC]
reversed_version590
reverse_efficiency-0.368[CC]
[edit]
Categories
//direction/forward
//terminator/single
Parameters
biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]