Coding

Part:BBa_K1342002

Designed by: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei   Group: iGEM14_Nagahama   (2014-10-03)
Revision as of 13:50, 16 October 2014 by Naoki (Talk | contribs) (TLC Assay)

AspA LVA(-)

Asp PDB.jpg
We removed LVA tag that renders protein susceptible to the action of tail-specific proteases from BBa_C0083 .
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 964
    Illegal AgeI site found at 493
    Illegal AgeI site found at 1345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1004



TLC Assay

fig.1
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)



fig.2
Estimation of aspartic acid content in spot Each intensity of spots indicating the content of aspartic acid, was estimated by using Image J. .
[edit]
Categories
//cds
//cds/enzyme
//function/biosynthesis
//function/motility
Parameters
directionForward
proteinAspA