Part:BBa_K1453000
ssDsbA-mRFP-Lgt-TAL USB-His Tag
This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.
Structure
The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
Design
When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.
With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning)
More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1158
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