Part:BBa_K1453000

ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of connectee.

The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL adapter also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI. When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`. With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning) More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 612
  Illegal AgeI site found at 724
  Illegal AgeI site found at 1537
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1158