Device

Part:BBa_K1475005:Design

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-10-05)
Revision as of 12:30, 16 October 2014 by Danie12 (Talk | contribs)

GFP controlled by TetR and pTet.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1390


Design Notes

This part was constructed using standard assembly from the iGEM registry parts Part:BBa_K1475004 and Part:BBa_E0840.


Source

TetR: ”Escherichia coli”
GFP: ”Aequeora victoria” (GFPmut3b) [3]


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
3. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850