Reporter

Part:BBa_J58101

Designed by: Valencia iGEM 2006   Group: iGEM06_Valencia   (2006-10-26)
Revision as of 11:02, 26 October 2006 by Guillermo (Talk | contribs)

Promoter activated synergistically by cI and CRP with the reporter RFP

A promoter activated synergistically by cI and CRP proteins (part ref. BBa_J58100) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal AgeI site found at 687
    Illegal AgeI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/pret
Parameters
None